陕西快乐十分最大遗漏数据
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動物行為學研究

睡眠剝奪系統

時間:2017-07-04來源:本站作者:玉研儀器

詳細介紹

大小鼠睡眠剝奪系統是一款不需要對動物進行訓練,以溫和的方式對動物進行睡眠限制的儀器。 參數設定完成后,不需要人為的干預即可對大鼠或小鼠進行睡眠剝奪的實驗



該系統有兩個型號:Feedback 和 StandAlone


Stand Alone型



可由電腦和控制器觸屏控制;

擊打棒以 5-15 RPM 的速度在鼠孔籠內轉動,并且可以通過程序編制改變方向,以減少睡眠剝奪的動物對環境的適應;


系統可提供食物、水和睡眠場所,動物籠分大鼠籠和小鼠籠;



 


Feedback 型



可以通過 EEG/EMG 腦電肌電系統實時監測的腦電肌電信號來進行睡眠剝奪,系統可以設置好動物的睡眠狀態,當實時監測的腦電肌電信號與預設置的睡眠狀態匹配或相似時,系統控制擊打棒轉動;

由電腦和控制器觸屏控制;

系統根據實時監測的腦電肌電信號控制擊打棒,擊打棒會以 5-15 RPM 的速度轉動,可以通過程序編制改變方向,以減少睡眠剝奪的動物對環境的適應;

系統提供食物、水和睡眠場所,動物籠分大鼠籠和小鼠籠;


     





FEEDBACK睡眠剝奪系統,STAND ALONE睡眠剝奪系統

我們提供兩種形式的自動睡眠剝奪系統:STAND ALONE睡眠剝奪系統和FEEDBACK睡眠剝奪系統。

FEEDBACK睡眠剝奪系統根據實時采集到動物的EEGEMG信號作為依據,反饋控制旋轉桿的動作。

FEEDBACK SLEEP DEPRIVATION SYSTEM

The FEEDBACK SYSTEM provides calendar-scheduling functionality plus the capability of adding real-time EEG/EMG feedback to ensure the bar rotates only when the animal enters a sleep-like state. Bar rotation starts and stops automatically based on user established rule sets for the animal’s sleep state, and users can easily incorporate delays, shifts in bar rotation, and time restrictions into the experimental setup.


The Feedback system can be controlled by a computer or by the programmable interface on the front panel of the device. Operating in feedback mode requires a computer connection.

  • The bar rotates at speeds between five and fifteen RPM and can be programmed to change directions to reduce acclimatization to the sleep deprivation environment. Bar rotation can be completely controlled by real-time EEG and EMG activity.
  • System accommodates water, food, and bedding. A variety of cage sizes are availble for mice and rats.
  • A Pinnacle EEG/EMG system is required to use EEG & EMG signals to regulate sleep deprivation. See the "Rules/Scheduling" tab below for more information.
  • Optional video recording provides a platform for synchronizing EEG and EMG changes with observable behavioral states. Captured video is displayed on screen and is synchronized with other recorded data in playback mode. 

RULES-BASED PROGRAMMING

The true versatility of the feedback system is unlocked in Sirenia? Acquisition with the included license key. Our software uses EEG and EMG data to establish thresholds, or rule sets, for the animal’s sleep patterns. Rule criteria can be easily adjusted by the researcher to target specific sleep behaviors and the system can be programmed to operate only during user-defined periods of time.

 

YOKED CONTROL FUNCTIONALITY

Add additional units to perform yoked control sleep studies. All systems can be programmed to operate simultaneously under one schedule or set of rules. A feedback system is required for yoked control.

EFFECTIVENESS OF YOKED CONTROL SLEEP DEPRIVATION

EEG and EMG activity were recorded for 24 hours (baseline) from two cohorts of five male C57BL/6J mice. During the following 24 hours, the first cohort was actively sleep deprived (SD) for a 12-hour lights-on/12-hour lights-off period. An identical cohort of five mice was subjected to yoked control (YC) sleep deprivation. The YC mice underwent the exact same sleep deprivation stimuli and duration as SD animals but were otherwise allowed to sleep ad lib. The chart below shows total cumulative sleep loss accrued during the 24-hour sleep deprivation period for both cohorts. Sleep loss was calculated by subtracting the total minutes of sleep accumulated during sleep deprivation from the total minutes of sleep accumulated during the same period during baseline. Purple bars represent the SD cohort, and gold bars represent the YC cohort. Vertical bars represent the standard error of the mean.

 

A Pinnacle EEG/EMG system is required to operate the sleep deprivation unit in feedback mode. Pinnacle offers three- and four-channel systems for biopotential recording. Tethered solutions are available for both mice and rats. 

 

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陕西快乐十分最大遗漏数据